Research Technician B

The Canesso Lab studies how intercellular communication in the intestine controls both physiological development and pathological disorders in early life and adulthood. We are interested in the gut-mammary gland axis and how maternal immune status during pregnancy and lactation impact offspring fitness. We want to better our understanding of tolerogenic and inflammatory responses developed in early life using a combination of innovative transgenic animal models and state-of-the-art genetic and chemical technology that we developed to track specific cell-cell interactions.

We especially seek team players with a strong sense of curiosity and drive to explore. As a key member of our team, the technician will play a crucial role in supporting our research and contributing to the understanding of the development and function of the intestinal and mammary gland immune systems. Specific projects focus on dissecting the immune responses against food, gut microbiota and cancer, as well as understanding both genetic and environmental drivers of intestinal immune phenotypes. The technician will use mouse models to assess gut and mammary gland immune cells by flow cytometry and RNA-sequencing and will be responsible for mouse colony maintenance. It is a primary role of the technician to handle the lab gnotobiotic mice. Other technical responsibilities may include bacterial and mammalian cell culture (including organoid cultures), ELISA, as well as molecular biology techniques such as cloning, RNA/DNA extractions, PCR and single-cell PCRs.

The person will:

• Perform a moderate number of simple or moderate technical procedures including: mouse ear punching, mouse weaning, mouse injections, mouse tissue harvesting, flow cytometry and  Immunoassays (e.g. ELISA)
• Require occasional guidance;
• Occasionally be responsible for ordering routine supplies;
• Rarely modify technical procedures;
• Rarely train other lab members;
• Rarely guide other lab members;
• A simple / limited understanding of scientific literature and the research goals and rationale is required;
• Occasionally analyze data;
• Rarely interpret data;
• Rarely make written contributions to papers and grant proposals.

Required to have at least a Bachelor’s Degree in a related science or equivalent skills and related experience, and zero to two years of related experience.

Preferred:

• Willing to handle mice.
• Experience with gnotobiotic mouse management. 
• Highly motivated with an interest in immunology.
• Well organized, demonstrate strong attention to detail, and be able to work effectively in a collaborative environment.